Test Details
Synonyms
- BCMA
- TNFRS17
- CD269
Limitations
Results of this test are labeled for research purposes only by the assay’s manufacturer. The performance characteristics of this assay have not been established by the manufacturer. The result should not be used for treatment or for diagnostic purposes without confirmation of the diagnosis by another medically established diagnostic product or procedure. The performance characteristics were determined by OncoTracker.
Methodology
Enzyme-linked immunosorbent assay (ELISA)
Reference Interval
Normal: 22.900 – 509.100 ng/mL
Reportable Range
3.906 – 8000.000 ng/mL
Additional Information
BCMA is a member of the tumor necrosis factor receptor superfamily (TNFRSF). It has been designated TNFRSF17. BCMA is a type III membrane protein containing one extracellular cysteine rich domain. Within the TNFRSF, it shares the highest homology with TACI. BCMA and TACI have both been shown to bind to A Proliferation-Inducing Ligand (APRIL) and B-Cell Activating Factor (BAFF), members of the TNF ligand superfamily. BCMA expression has been found in immune organs and mature B cell lines. Although some expression has been observed at the cell surface, BCMA appears to be localized to the Golgi compartment. The binding of BCMA to APRIL or BAFF has been shown to stimulate IgM production in peripheral blood B cells and increase the survival of cultured B cells. This data suggests that BCMA may play an important role in B cell development, function and regulation. Human BCMA is a 184 amino acid (aa) protein consisting of a 54 aa extracellular domain, a 23 aa transmembrane domain, and a 107 aa intracellular domain. Mouse and human BCMA share 62% amino acid identity. (1)
Specimen Requirements
Specimen Type
Serum
Volume
0.5 mL
Minimum Volume
0.1 mL
Container
Red-top tube
Serum separating tube (SST)
Cryovial
Collection
Whole Blood Collection
- Fully fill a red top tube (volume dependent on study/testing needs)
- Allow tube of blood to clot at room temperature for 30 minutes in an upright, vertical position
- If not centrifuged immediately after clotting period, store tube at 2-8°C for no more than 4 hours prior to processing
Serum Collection
- Centrifuge tube for 10 minutes at 1000 RCF
- Pipette serum from the top layer and transfer into a cryo storage tube
- Label storage tube
- Freeze storage tube (@ -80°C) within 15 minutes of aliquoting
- Discard the red top tubes and remaining specimens as medical waste
Storage Instructions
Freeze
Stability
| Temperature | Period |
| Frozen (-80°C) | 182 days* |
| Refrigerated (2-8°C) | 14 Days |
| Room Temp (20-25°C) | 21 Days |
| Freeze/thaw cycles | 8 cycles |
| *Frozen stability evaluations are ongoing | |
Causes for Rejection
Non-serum specimen; gross lipemia; gross hemolysis; inadequate testing volume
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References
- IFU; Human BCMA/TNFRSF17 DuoSet ELISA kit, Catalog Number: DY193, R&D Systems
